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Primary antibodies were diluted in BB and labeled at 4C overnight

The Increlex (mecasermin) FDA label, which covers native rhIGF-1 in pediatric severe primary IGFD, is the closest human reference and informs the contraindications below

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Glutamine, a metabolic component of essential amino acids, plays a key role in CRC growth, further highlighting the importance and necessity of glutamine in tumor cell metabolism

Consequently, the balance between system Xc and glutamate transport is disrupted, leading to both a reduced intracellular uptake of glutamate and an inhibition of GSH synthesis due to the lack of necessary raw materials (87)